*Correspondence to: Marbel Torres Arias, Laboratorio de Inmunología y Virología, CENCINAT, GISAH, Universidad de las Fuerzas Armadas, Sangolquí, Pichincha, Ecuador
Received: Sep 17, 2022; Accepted: Sep 23, 2022; Published: Sep 30, 2022
Citation: David C, Torres Arias M (2022) Cell-SELEX as an Approach to Develop Aptamer Molecules to Recognize Leishmania mexicana. J Nanomed Nanotech Nanomat. 3: 118.
Copyright: ©2022 Torres Arias M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT
The recognition of pathogen is a basic first step needed to move forward a treatment approach. Therefore, novel ways to identify parasites of Leishmania mexicana are needed to change the drawbacks of currents treatments. Aptamers are single stranded DNA or RNA molecules capable of target a specific protein or cell created during SELEX cycles. The aim of this investigation is to obtain DNA aptamers that recognize specifically Leishmania mexicana parasites using in silico approaches and Cell-SELEX technique for further use in the Leishmaniasis treatment. The cycles used to perform Cell-SELEX consist of exposure of the parasite culture to a DNA pool, binding, recovery and amplification of the bound sequences to the target through asymmetric PCR to recover back ssDNA pools to act as an aptamer for the parasite. Using variation of the SELEX conditions during every cycle created a robust aptamer pool able to recognize specifically Leishmania mexicana.
Keywords: Aptamers; Cell-SELEX; Leishmania mexicana
GRAPHICAL ABSTRACT
